Table 1 Optimization results for the AuNP-mAb probe volume, sample volume and chromatographic time
group | AuNPs probe volume (μL) | sample volume (μL) | chromatographic time (min) | signal intensity of negative samples | signal intensity of positive samples |
|---|---|---|---|---|---|
1 | 2 | 100 | 3 | + | - |
2 | 2 | 150 | 3 | + | - |
3 | 2 | 200 | 3 | + + | - |
4 | 4 | 100 | 5 | + + | + |
5 | 4 | 150 | 5 | + + + | - |
6 | 4 | 200 | 5 | + + + + | + |
7 | 6 | 100 | 8 | + + + + | + |
8 | 6 | 150 | 8 | + + + + | + + |
9 | 6 | 200 | 8 | + + + + + | + + |