Fig. 3

Expression and depolymerization activity of K62-ORF30 depolymerase. Purified K62-Dpo30 protein was determined by 10% SDS-PAGE gel and Coomassie blue staining. B The polysaccharide depolymerization activity of K62-Dpo30 protein was examined through the spot assay utilizing various concentrations (ranging from 0.1 mg/mL to 1.7 mg/mL) of K62-Dpo30 protein. SUMO protein was employed as a negative control. C The impact of K62-Dpo30 depolymerase on K62-CPS was assessed by SEC-HPLC. The K62-CPS was incubated with either 10μg/mL K62-Dpo30 protein for 30 min at 37 °C (blue line), or at 25 °C (green line), or with 10μ g/mL SUMO protein for 30 min at 37 °C (red line). D The effect of K62-Dpo30 on K62-CPS was analyzed by Alcian blue staining. K62-CPS was incubated with or without K62-ORF30 or SUMO protein for 30 min at 25 °C