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Fig. 3 | One Health Advances

Fig. 3

From: Preparation of a highly specific and sensitive monoclonal antibody against tilmicosin and its application in lateral flow immunoassay

Fig. 3

LFA development. Optimization of A antigen concentration, B mAb 12C8 usage, C volume of ACNPs-mAb 12C8 of ACNPs-LFA, respectively. Optimization of D antigen concentration, E mAb 12C8 usage, F volume of CGNPs-mAb 12C8 of CGNPs-LFA, respectively. G Images of developed LFA. H Calibration curves of ACNPs-LFA and CGNPs-LFA I Specificity of ACNPs-LFA. 1 = PBST; 2 = TM at 1 ng/mL; 3, 4, 5, 6, 7 and 8 = tylosin, tiamulin, avermectin, streptomycin, erythromycin and spiramycin at 100 ng/mL, respectively

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