Table 1 Procedure of MVA titration by plaque assay using crystal violet staining in DF-1 cells

1) Seed DF-1 cells in 24-well plate until confluence in DMEM containing 10% FBS

2) Infect the cells with MVA using a series of dilutions using DMEM containing 2.5% FBS

3) One hour after infection, replace the media with DMEM containing 2.5% FBS and 0.5% methylcellulose

4) After 48 h of incubation, fix and stain DF-1 cells with a solution of 20% methanol and 0.1% crystal violet for 20 min

5) Wash the wells, visualize and count the plaques