The replicase protein nsp2 of Chinese highly pathogenic porcine reproductive and respiratory virus is involved in protective immunity by promoting viral clearance

Table 2 Primers used for construction of plasmids coding for nsp2 derivatives and nsp9

Primer name	Sequence (5’-3’)
nsp2 (aa.12–323/521/782)_JX-F	ACTTTAGAGGAGATATACCATGGGCGCGACTACTATGGTCGCTCATCACGCT
nsp2 (aa.12–521)_JX-R	GAACTGCGGGTGGCTCCAGGTACCTGCCGACCCACTCAAAGGTGTCATCAG
nsp2 (aa.12–782)_JX-R	GAACTGCGGGTGGCTCCAGGTACCGCC GACACCACCTGTATCTGCGGGCGG
nsp2 (aa.12–323)_JX-R	GAACTGCGGGTGGCTCCAGGTACCGCCCAAAGGCTCTTGAGTCACGGG
nsp9_JX-F	ACTTTAGAGGAGATATACCATGGGCTTTAAACCTGCTAGCCGCCAGCGG
nsp9_JX-R	GAACTGCGGGTGGCTCCAGGTACCGCCCTCATGATTGGACCTGAGTTTTTC

ISSN: 2731-9970