The replicase protein nsp2 of Chinese highly pathogenic porcine reproductive and respiratory virus is involved in protective immunity by promoting viral clearance

Kong, Can; Li, Dan; Hu, Yanxin; Gao, Peng; Zhang, Yongning; Zhou, Lei; Ge, Xinna; Guo, Xin; Han, Jun; Yang, Hanchun

doi:10.1186/s44280-023-00026-8

Table 1 Primers used for construction of infectious cDNA clones and viral RNA quantification

From: The replicase protein nsp2 of Chinese highly pathogenic porcine reproductive and respiratory virus is involved in protective immunity by promoting viral clearance

Primer name	Sequence (5'-3')	Usage
UP-PLP2_SX-1F	AGCTGGTTTAGTATTTAAATACCGTCTATGACGTATAGGTGTTG (*Swa*I)	Construction of chimeric cDNA clones CHsx1401-PLP2_JX
UP-PLP2_SX-1R	TGCTCTCTTTCCGGCACCATACCACTTATGGACGCCAAA
PLP2_JX-2F	CATAAGTGGTATGGTGCCGGAAAGAGAGCAAGGAAACCA
PLP2_JX-2R	TCGAGACTGAAATTCGCCCAAAGGCTCTTGAGTCACGGG
Down-PLP2_SX-3F	CAAGAGCCTTTGGGCGAATTTCAGTCTCGAAAAGCGGAG
Down-PLP2_SX-3R	CTACAGAGAGCACCGGTGTGTGGGGCATCATCAC (*SgrA*I)
UP-PLP2_JX-1F	CAGAGCTGGTTTAGTATTTAAATACCGTCATGACGTATAGGTGT (*Swa*I)	Construction of chimeric cDNA clones JXwn06-PLP2_SX
UP-PLP2_JX-1R	ACCGTACCACTTATGACTGCCAAATGTTCTCTTCCCAGC
PLP2_SX-2F	CATAAGTGGTACGGTGCTGGGAAGAGAACAAGAAAAGCA
PLP2_SX-2R	AGGGACCGAGTCCTTCGGAGACCTTTGGGCCACGAC
Down-PLP2_JX-3F	CAAAGGTCTCCGCCGAAGGACTCGGTCCCTCTGACCGCC
Down-PLP2_JX-3R	GCCTCCCCCTGAAGGCTTCGAAATTTGCCTGATCTTTAGTCCATT (*Bst*I)
UP-SP_JX-1F	TTACAATGATGCGTTTCGGGCGCGCCAGAAAGGGAAAAT (*Asc*I)	Construction of chimeric cDNA clones JXwn06-SP_SX
UP-SP_JX-1R	TGGGCTGGCATTCTTTGGCACCTC
SP_SX-2F	TAGGCCTGAATTGAAATGAAATGGGGGCTTTGCAGAGCA
SP_SX-2R	AAGAATGCCAGCCCATCATGCTGGGGGTGACGTCGTGAC
Down-SP_JX-3F	GTCACCCCCAGCATGATGGGCTGGCATTCTTTGGCACCTC
Down-SP_JX-3R	GGACCATGCCGGCCTTAATTAATTTTTTTTTTTTTTTT (*Pac*I)
UP-SP_SX-1F	CAATGAAGCGTTTCGTGCGCGCCAGAAAGGGAAGATTTATAGAGCCA (*Asc*I)	Construction of chimeric cDNA clones CHsx1401-SP_JX
UP-SP_SX -1R	TAGACCCCATTTCATTTCAGTTCAAGCCTAAAGTTGGTT
SP_JX-2F	TAGGCTTGAACTGAAATGAAATGGGGTCTATGCAAAGCC
SP_JX-2R	AAGAATGCCAGCTCATCATGCTGAGGGTGATGCTGTGGC
Down-SP_SX-3F	TCACCCTCAGCATGATGAGCTGGCATTCTTGAGACATCC
Down-SP_SX-3R	GGGACCATGCCGGCCTTAATTAATTTTTTTTTTTTTTTTTTTTTTTT (*Pac*I)
Nsp9-CHsx1401-qPCR-F	TTCTGCGGTTCCTTCGCTTG	amplification of CHsx1401 nsp9
Nsp9-CHsx1401-qPCR-R	TCCTCTTTGGTGGCTTCGGA
Nsp9-CHsx1401-probe	FAM-CCCAAGTTACTTGCCCGC-MGB
nsp9-JXwn06-F	CTGCGATCGATCCACACCTG	amplification of JXwn06 nsp9
nsp9-JXwn06-R	GCGTGACCAGTAAGTCGTGG
nsp9-JXwn06-probe	VIC-TTTGCCGCCAATCTTCTT-MGB

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ISSN: 2731-9970

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