Table 1 Testing conditions for Enterobacterales and Actinobacillus spp. according to the CLSI document VET01S [3]
Bacteria | Test parameters | Details |
|---|---|---|
Enterobacterales | Medium | Disk diffusion: Mueller–Hinton agar |
Broth dilution: cation-adjusted Mueller–Hinton broth | ||
Agar dilution: Mueller–Hinton agar | ||
Inoculum | Broth culture method or colony suspension method, equivalent to a 0.5 McFarland standard | |
Incubation | 35 °C ± 2 °C; ambient air | |
Disk diffusion: 16 − 18 h | ||
Broth dilution: 16 − 20 h | ||
Agar dilution: 16 − 20 h | ||
Actinobacillus spp. | Medium | Disk diffusion: chocolate Mueller–Hinton agar |
Broth dilution: MHF-Y broth | ||
Agar dilution: chocolate Mueller–Hinton agar | ||
Inoculum | Colony suspension method, equivalent to a 0.5 McFarland standard using colonies from an overnight (18–24 h) culture on a chocolate agar plate incubated in 5% CO2 | |
Incubation | 35 °C ± 2 °C; 5% CO2 | |
Disk diffusion: 20 − 24 h | ||
Broth dilution: 20 − 24 h | ||
Agar dilution: 20 − 24 h |