Fig. 5

Protection against challenge strain of M. paratuberculosis. Following challenge, mice groups (n = 5) were sacrificed at 8 weeks after challenge and bacterial burden was analyzed in liver A and spleen B organs. The proportion of liver C and spleen D weight to total body weight was analyzed at 8 weeks after MAP infection. Following the challenge, mice groups (n = 5) were sacrificed at 12 weeks after the challenge, and bacterial burden was analyzed in the liver E and spleen F organs. The proportion of liver G and spleen H weight to total body weight was analyzed 12 weeks after MAP infection. A sterisk refers to statistical significant difference, *p < 0.05, **p < 0.01, and ***p < 0.001