Fig. 4
From: Bfra-loaded nanoparticles confer protection against paratuberculosis infection
Analysis of immune responses in immunized mice before challenge. Mice (n = 5) from each group were euthanized 2 weeks post-immunization to analyze the immune response. ELISA was used to detect A IL-10, B TNF-α, and C IgG in serum samples. Splenocytes were extracted and activated in vitro with Bfra to assess D IFN-γ. The graph of E CD4+ T cells and F CD8+ T cells in post-immunization spleen tissue of mice. A asterisk denotes a statistically significant difference: *p < 0.05, **p < 0.01, and ***p < 0.001