Fig. 3
From: Bfra-loaded nanoparticles confer protection against paratuberculosis infection
Experimental design for vaccination and challenge. Female C57BL/6 mice (6-8 weeks old) were subcutaneously vaccinated and then intraperitoneally challenged with a virulent strain of MAP 2015WD-1 two weeks later. At various times, mice (n = 5/group) were slaughtered. Tissue and blood samples were taken to determine the bacterial burden, cytokine levels, and histopathology