Fig. 1
From: Bfra-loaded nanoparticles confer protection against paratuberculosis infection
Preparation and characterization of Bfra NPs. A Western blotting with an anti-His monoclonal antibody confirmed the recombinant Bfra protein and protein retrieved from Bfra-PLGA NPs. B Purified recomniant Bfra was ~ 30 kDa, as measured by SDS-PAGE. C Scanning Electron Microscope (SEM) at magnifications of (C) × 6 k and D × 80 k for Bfra-NPs. E Average size and F potential of Bfra-NPs. G Residual Bfra (%) within Bfra-NPs at pH 7.4 and 5.8 was measured by BCA over 10 days’ time period