Re-emergence of canine Leishmania infantum infection in mountain areas of Beijing

Table 5 Primers used for gene sequencing and PCR

Target gene	Primer name and sequence	Y (T_A)℃	Gene length(bp)	Product size (bp)	Chromosome	Reference
alat	ALAT.F: GTGTGCATCAACCCMGGGAA	55	1494	589	12	Marco et al., 2015 [8]
alat	ALAT.R: CGTTCAGCTCCTCGTTCCGC
enol	ENOL.F: GCTGCCGATCCTGATGGAGG	55	12,090	431	14	Marco et al., 2015 [8]
enol	ENOL.R: ACCCGTTCTCCATGCACAGC
pgm	PGM.F: CAGAGAAGCTGACGTCCCAG	61	1770	529	21	Marco et al., 2015 [8]
pgm	PGM.R: GACGGGTTCACGAAGAAGCG
spdsyn	SPDSYN.F: CGAACCTGTCGCTGACGTG	55	903	394	4	Marco et al., 2015 [8]
spdsyn	SPDSYN.R: GAYTCGCCCTGGTTGCACAC
g6pdh	G6PDH.F: GTGGACTTCAAGCGTCTYGAC	61	1689	573	34	Lauthier et al., 2020 [21]
g6pdh	G6PDH.R: TCGGCGGAGGCRGTGTACTG
mpi	MPI.F: TAAGTACAAGGAYCCRAACCACAAG	61	1266	441	32	Lauthier et al., 2020 [21]
mpi	MPI.R: GCCATGATCTCRACACCGTC
icd	ICD.F: TCAACCTVAAGATGTGGAAGAG	61	1308	702	10	Lauthier et al., 2020 [21]
icd	ICD.R: GTCCAGGCGTAGATGGAGG