Table 1 Characterization of mice antisera and mAbs with homologous and heterologous coating antigens
Haptens/Mice# | Coating antigen Hapten A-BSAa | Coating antigen Hapten B-BSA | ||||
|---|---|---|---|---|---|---|
Antibody titers | IC50 for OXZ (ng mL−1) | IC50 for NPOXZ (ng mL−1) | Antibody titers | IC50 for OXZ (ng mL−1) | IC50 for NPOXZ (ng mL−1) | |
Hapten-A-1# | 1/15000 | 375.5 | 133.2 | 1/10000 | 121.2 | 89.3 |
Hapten-A-2# | 1/15000 | 256.3 | 143.5 | 1/12000 | 189.7 | 96.6 |
Hapten-A-3# | 1/14000 | 461.5 | 122.7 | 1/14000 | 211.4 | 71.2 |
Hapten-A-4# | 1/13000 | 353.9 | 126.1 | 1/10000 | 135.2 | 68.5 |
Hapten-A-5#b | 1/18000 | 519.1 | 189.3 | 1/16000 | 88.1 | 47.6 |
Average valuesc | 1/15000 | 393.26 | 142.96 | 1/12400 | 149.12 | 74.64 |
mAb 2B6 | 1/81000 | 1.89 | 1.46 | 1/81000 | 1.42 | 0.95 |
Hapten-B-1# | 1/20000 | 50.5 | 31.3 | 1/11000 | 278.3 | 148.9 |
Hapten-B-2# | 1/27000 | 28.1 | 12.4 | 1/20000 | 158.3 | 112.6 |
Hapten-B-3# | 1/20000 | 98.2 | 68.3 | 1/19000 | 198.6 | 125.8 |
Hapten-B-4# | 1/20000 | 156.2 | 65.6 | 1/15000 | 176.5 | 123.2 |
Hapten-B-5# | 1/20000 | 133.1 | 46.3 | 1/15000 | 146.4 | 126.1 |
Average values | 1/21400 | 93.22 | 44.78 | 1/16000 | 191.62 | 127.32 |
mAb 2D9 | 1/81000 | 0.66 | 0.21 | 1/81000 | 0.98 | 0.61 |